Generator

Part:BBa_K093017:Design

Designed by: John Heil   Group: iGEM08_Waterloo   (2009-04-20)


Pconst. phiC31 integrase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1726
    Illegal SapI.rc site found at 1417
    Illegal SapI.rc site found at 1475
    Illegal SapI.rc site found at 1580


Design Notes

see BBa_K093015 for information on ORF. This enzyme is required to be expressed. Inducible expression is not required. Constitutive expression simplifies use of the part, and reduces conflicts in future designs.


Source

see BBa_K093015 for information on ORF

References